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Princeton HPLC Columns  
Princeton Chiral Columns  
The chiral phases, DNB-LEU or DNB-PHGLY, are prepared from N-3-5-dinitrobenzoyl protected derivatives of leucine or phenylgylcine, (which are then bonded to silica stationary phase). These phases are available in both the D- or the L- enantiomers of the amino acids.
The unique feature of amino-acid-derived chiral phases is that the elution order of enantiomers in the sample is dependent on the D- or L- chirality of the bonded amino acid. Therefore, the order of elution can be controlled by appropriate selection of stationary phase. This can be of tremendous advantage for those samples which contain a large excess of one enantiomer, in which case it is desirable to have the minor component elute first.
Many racemic acids and amines which cannot be resolved in their native form can be easily converted to amides or anilides which can be resolved by an amino-acid-derived chiral phase. The following compounds are candidates for amino-acid-derived chiral phases:
Aromatic alcohols Aryl substituted phthalides
Aromatic amino acids Aryl substituted lactams
Aromatic amino alcohols Aryl substituted succinamides
Aromatic thiols Aryl substituted hydantoins
Aromatic amines Aryl substituted hydantoins
Aromatic sulfoxides Aryl substituted hydantoins
Mandelic acid analogs Aryl substituted hydantoins
Phase: D-DNB-LEU  
Part # Length x ID Particle Size Price Buy
250046-03511 250 x 4.6 mm 5 µm $700.00
150046-03511 150 x 4.6 mm 5 µm $675.00
150046-03311 150 x 4.6 mm 3 µm $750.00
100046-03311 100 x 4.6 mm 3 µm $700.00
250040-03511 250 x 4.0 mm 5 µm $700.00
150040-03511 150 x 4.0 mm 5 µm $675.00
150040-03311 150 x 4.0 mm 3 µm $750.00
100040-03311 100 x 4.0 mm 3 µm $700.00
250030-03511 250 x 3.0 mm 5 µm $700.00
150030-03511 150 x 3.0 mm 5 µm $675.00
150030-03311 150 x 3.0 mm 3 µm $750.00
100030-03311 100 x 3.0 mm 3 µm $700.00
250020-03511 250 x 2.0 mm 5 µm $750.00
150020-03511 150 x 2.0 mm 5 µm $700.00
150020-03311 150 x 2.0 mm 3 µm $775.00
100020-03311 100 x 2.0 mm 3 µm $725.00
Phase: L-DNB-LEU  
Part # Length x ID Particle Size Price Buy
250046-03512 250 x 4.6 mm 5 µm $700.00
150046-03512 150 x 4.6 mm 5 µm $675.00
150046-03312 150 x 4.6 mm 3 µm $750.00
100046-03312 100 x 4.6 mm 3 µm $700.00
250040-03512 250 x 4.0 mm 5 µm $700.00
150040-03512 150 x 4.0 mm 5 µm $675.00
150040-03312 150 x 4.0 mm 3 µm $750.00
100040-03312 100 x 4.0 mm 3 µm $700.00
250030-03512 250 x 3.0 mm 5 µm $700.00
150030-03512 150 x 3.0 mm 5 µm $675.00
150030-03312 150 x 3.0 mm 3 µm $750.00
100030-03312 100 x 3.0 mm 3 µm $700.00
250020-03512 250 x 2.0 mm 5 µm $750.00
150020-03512 150 x 2.0 mm 5 µm $700.00
150020-03312 150 x 2.0 mm 3 µm $775.00
1000206-03312 100 x 2.0 mm 3 µm $725.00
Phase: D-DNB-PHGLY  
Part # Length x ID Particle Size Price Buy
250046-03513 250 x 4.6 mm 5 µm $700.00
150046-03513 150 x 4.6 mm 5 µm $675.00
150046-03313 150 x 4.6 mm 3 µm $750.00
100046-03313 100 x 4.6 mm 3 µm $700.00
250040-03513 250 x 4.0 mm 5 µm $700.00
150040-03513 150 x 4.0 mm 5 µm $675.00
150040-03313 150 x 4.0 mm 3 µm $750.00
100040-03313 100 x 4.0 mm 3 µm $700.00
250030-03513 250 x 3.0 mm 5 µm $700.00
150030-03513 150 x 3.0 mm 5 µm $675.00
150030-03313 150 x 3.0 mm 3 µm $750.00
100030-03313 100 x 3.0 mm 3 µm $700.00
250020-03513 250 x 2.0 mm 5 µm $750.00
150020-03513 150 x 2.0 mm 5 µm $700.00
150020-03313 150 x 2.0 mm 3 µm $775.00
100020-03313 100 x 2.0 mm 3 µm $725.00
Phase: L-DNB-PHGLY  
Part # Length x ID Particle Size Price Buy
250046-03514 250 x 4.6 mm 5 µm $700.00
150046-03514 150 x 4.6 mm 5 µm $675.00
150046-03314 150 x 4.6 mm 3 µm $750.00
100046-03314 100 x 4.6 mm 3 µm $700.00
250040-03514 250 x 4.0 mm 5 µm $700.00
150040-03514 150 x 4.0 mm 5 µm $675.00
150040-03314 150 x 4.0 mm 3 µm $750.00
100040-03314 100 x 4.0 mm 3 µm $700.00
250030-03514 250 x 3.0 mm 5 µm $700.00
150030-03514 150 x 3.0 mm 5 µm $675.00
150030-03314 150 x 3.0 mm 3 µm $750.00
100030-03314 100 x 3.0 mm 3 µm $700.00
250020-03514 250 x 2.0 mm 5 µm $750.00
150020-03514 150 x 2.0 mm 5 µm $700.00
150020-03314 150 x 2.0 mm 3 µm $775.00
100020-03314 100 x 2.0 mm 3 µm $725.00


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